3alfa(o 20beta)-idrossisteroide deidrogenasi
enzima
La 3alfa(o 20beta)-idrossisteroide deidrogenasi è un enzima appartenente alla classe delle ossidoreduttasi, che catalizza la seguente reazione:
| 3alfa(o 20beta)-idrossisteroide deidrogenasi | |
|---|---|
 Modello tridimensionale dell'enzima | |
| Numero EC | 1.1.1.53 |
| Classe | Ossidoreduttasi |
| Nome sistematico | |
| 3α(or 20β)-hydroxysteroid:NAD+ oxidoreductase | |
| Altri nomi | |
| cortisone reduttasi; (R)-20-idrossisteroide deidrogenasi; Δ4-3-chetosteroide deidrogenasi; 20β-idrossisteroide deidrogenasi; NADH-20β-idrossisteroide deidrogenasi; 20β-HSD | |
| Banche dati | BRENDA, EXPASY, GTD, PDB (RCSB PDB PDBe PDBj PDBsum) |
| Fonte: IUBMB | |
- androstano-3α,17β-diolo + NAD+ ⇄ 17β-idrossiandrostene-3-one + NADH + H+
I gruppi ossidrile in posizione 3α- e 20β- del pregnano e dell'androstano possono agire come donatori
Bibliografia
modifica- Edwards, C.A.F. and Orr, J.C., Comparison of the 3α-and 20β-hydroxysteroid dehydrogenase activities of the cortisone reductase of Streptomyces hydrogenans, in Biochemistry, vol. 17, 1978, pp. 4370–4376, Entrez PubMed 718844.
- Hübener, H.J. and Sahrholz, F.G., 20β-hydroxy-steroid-dehydrogenase. II. Darstellung und Kristallisation, in Biochem. Z., vol. 333, 1960, pp. 95–105, Entrez PubMed 16526091.
- Hübener, H.J., Sahrholz, F.G., Schmidt-Thomé, J., Nesemann, G. and Junk, R., 20β-Hydroxy-Steroid-Dehydrogenase, ein neues kristallines Enzym, in Biochim. Biophys. Acta, vol. 35, 1959, pp. 270–272, Entrez PubMed 16526091.
- Lynn, W.S. and Brown, R.H., The conversion of progesterone to androgens by testes, in J. Biol. Chem., vol. 232, 1958, pp. 1015–1030, Entrez PubMed 13549484.
- Strickler, R.C., Covey, D.F. and Tobias, B., Study of 3α, 20 β-hydroxysteroid dehydrogenase with an enzyme-generated affinity alkylator: dual enzyme activity at a single active site, in Biochemistry, vol. 19, 1980, pp. 4950–4954, Entrez PubMed 6936053.
- Sweet, F. and Samant, B.S., Bifunctional enzyme activity at the same active site: study of 3α and 20β activity by affinity alkylation of 3α, 20β-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids, in Biochemistry, vol. 19, 1980, pp. 978–986, Entrez PubMed 6928375.
